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Journal: Cell & Bioscience
Article Title: USP43 promotes cerebral ischemia–reperfusion injury via activation of TAK1
doi: 10.1186/s13578-025-01475-x
Figure Lengend Snippet: USP43 regulates the TAK1-JNK/p38 signaling pathway during cerebral I–R injury . a Western blotting showed total and phosphorylation levels of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38 in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. b Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. c Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations, n.s. p > 0.05. d Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. e Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. f Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. #p < 0.05, ##p < 0.01 vs. WT Sham, AdshRNA Control or Ad Gfp Control group, and n.s. p > 0.05, **p < 0.01 vs. WT I–R, AdshRNA OGD/R or Ad Gfp OGD/R group
Article Snippet:
Techniques: Western Blot, Phospho-proteomics, Infection, Cell Culture, Control