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94
Proteintech p ask1
USP43 regulates the TAK1-JNK/p38 signaling pathway during cerebral I–R injury . a Western blotting showed total and phosphorylation levels of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38 in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. b Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. c Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations, n.s. p > 0.05. d Western blotting showed total and phosphorylation levels of TAK1 and <t>Ask1</t> in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. e Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. f Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. #p < 0.05, ##p < 0.01 vs. WT Sham, AdshRNA Control or Ad Gfp Control group, and n.s. p > 0.05, **p < 0.01 vs. WT I–R, AdshRNA OGD/R or Ad Gfp OGD/R group
P Ask1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cell Signaling Technology Inc western blotting-related antibodies α-sma, il-1β, bax, cleaved caspase-3, ask1, p-ask1, p38, p-p38, jnk, p-jnk, and c-jun
USP43 regulates the TAK1-JNK/p38 signaling pathway during cerebral I–R injury . a Western blotting showed total and phosphorylation levels of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38 in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. b Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. c Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations, n.s. p > 0.05. d Western blotting showed total and phosphorylation levels of TAK1 and <t>Ask1</t> in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. e Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. f Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. #p < 0.05, ##p < 0.01 vs. WT Sham, AdshRNA Control or Ad Gfp Control group, and n.s. p > 0.05, **p < 0.01 vs. WT I–R, AdshRNA OGD/R or Ad Gfp OGD/R group
Western Blotting Related Antibodies α Sma, Il 1β, Bax, Cleaved Caspase 3, Ask1, P Ask1, P38, P P38, Jnk, P Jnk, And C Jun, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ZenBio p-ask1 protein no: 310216
USP43 regulates the TAK1-JNK/p38 signaling pathway during cerebral I–R injury . a Western blotting showed total and phosphorylation levels of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38 in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. b Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. c Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations, n.s. p > 0.05. d Western blotting showed total and phosphorylation levels of TAK1 and <t>Ask1</t> in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. e Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. f Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. #p < 0.05, ##p < 0.01 vs. WT Sham, AdshRNA Control or Ad Gfp Control group, and n.s. p > 0.05, **p < 0.01 vs. WT I–R, AdshRNA OGD/R or Ad Gfp OGD/R group
P Ask1 Protein No: 310216, supplied by ZenBio, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology anti phospho ask1
USP43 regulates the TAK1-JNK/p38 signaling pathway during cerebral I–R injury . a Western blotting showed total and phosphorylation levels of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38 in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. b Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. c Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations, n.s. p > 0.05. d Western blotting showed total and phosphorylation levels of TAK1 and <t>Ask1</t> in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. e Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. f Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. #p < 0.05, ##p < 0.01 vs. WT Sham, AdshRNA Control or Ad Gfp Control group, and n.s. p > 0.05, **p < 0.01 vs. WT I–R, AdshRNA OGD/R or Ad Gfp OGD/R group
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Proteintech anti p ask1
USP43 regulates the TAK1-JNK/p38 signaling pathway during cerebral I–R injury . a Western blotting showed total and phosphorylation levels of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38 in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. b Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. c Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations, n.s. p > 0.05. d Western blotting showed total and phosphorylation levels of TAK1 and <t>Ask1</t> in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. e Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. f Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. #p < 0.05, ##p < 0.01 vs. WT Sham, AdshRNA Control or Ad Gfp Control group, and n.s. p > 0.05, **p < 0.01 vs. WT I–R, AdshRNA OGD/R or Ad Gfp OGD/R group
Anti P Ask1, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biorbyt polyclonal p ask1
USP43 regulates the TAK1-JNK/p38 signaling pathway during cerebral I–R injury . a Western blotting showed total and phosphorylation levels of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38 in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. b Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. c Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations, n.s. p > 0.05. d Western blotting showed total and phosphorylation levels of TAK1 and <t>Ask1</t> in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. e Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. f Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. #p < 0.05, ##p < 0.01 vs. WT Sham, AdshRNA Control or Ad Gfp Control group, and n.s. p > 0.05, **p < 0.01 vs. WT I–R, AdshRNA OGD/R or Ad Gfp OGD/R group
Polyclonal P Ask1, supplied by Biorbyt, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Affinity Biosciences anti-p-ask1 no.af3477
USP43 regulates the TAK1-JNK/p38 signaling pathway during cerebral I–R injury . a Western blotting showed total and phosphorylation levels of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38 in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. b Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. c Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations, n.s. p > 0.05. d Western blotting showed total and phosphorylation levels of TAK1 and <t>Ask1</t> in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. e Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. f Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. #p < 0.05, ##p < 0.01 vs. WT Sham, AdshRNA Control or Ad Gfp Control group, and n.s. p > 0.05, **p < 0.01 vs. WT I–R, AdshRNA OGD/R or Ad Gfp OGD/R group
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Cell Signaling Technology Inc anti p ask1
USP43 regulates the TAK1-JNK/p38 signaling pathway during cerebral I–R injury . a Western blotting showed total and phosphorylation levels of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38 in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. b Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. c Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations, n.s. p > 0.05. d Western blotting showed total and phosphorylation levels of TAK1 and <t>Ask1</t> in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. e Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. f Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. #p < 0.05, ##p < 0.01 vs. WT Sham, AdshRNA Control or Ad Gfp Control group, and n.s. p > 0.05, **p < 0.01 vs. WT I–R, AdshRNA OGD/R or Ad Gfp OGD/R group
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USP43 regulates the TAK1-JNK/p38 signaling pathway during cerebral I–R injury . a Western blotting showed total and phosphorylation levels of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38 in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. b Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. c Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations, n.s. p > 0.05. d Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. e Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. f Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. #p < 0.05, ##p < 0.01 vs. WT Sham, AdshRNA Control or Ad Gfp Control group, and n.s. p > 0.05, **p < 0.01 vs. WT I–R, AdshRNA OGD/R or Ad Gfp OGD/R group

Journal: Cell & Bioscience

Article Title: USP43 promotes cerebral ischemia–reperfusion injury via activation of TAK1

doi: 10.1186/s13578-025-01475-x

Figure Lengend Snippet: USP43 regulates the TAK1-JNK/p38 signaling pathway during cerebral I–R injury . a Western blotting showed total and phosphorylation levels of c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK) and p38 in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. b Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. c Western blotting showed the phosphorylation levels and total protein levels of JNK, ERK and p38 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations, n.s. p > 0.05. d Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in WT and Usp43 -KO mice at 24 h after I–R. n = 3 mice per group. e Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with AdshRNA and Adsh Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. f Western blotting showed total and phosphorylation levels of TAK1 and Ask1 in primary neurons infected with Ad Gfp and Ad Usp43 at 6 h after OGD/R. n = 3 of independent cell culture preparations. #p < 0.05, ##p < 0.01 vs. WT Sham, AdshRNA Control or Ad Gfp Control group, and n.s. p > 0.05, **p < 0.01 vs. WT I–R, AdshRNA OGD/R or Ad Gfp OGD/R group

Article Snippet: p-ASK1 , 28846-1-AP , Proteintech.

Techniques: Western Blot, Phospho-proteomics, Infection, Cell Culture, Control